Journal: Microbiology Spectrum

Article Title: Ability of Two Strains of Lactic Acid Bacteria To Inhibit Listeria monocytogenes by Spot Inoculation and in an Environmental Microbiome Context

doi: 10.1128/spectrum.01018-22

Figure Lengend Snippet: Bacterial strains used in this study

Article Snippet: Two lactic acid bacterium strains previously identified by Zhao et al. ( ) as Enterococcus durans 152 (PS01156; ATCC PTA-4759) and Lactococcus lactis subsp. lactis C-1-152 (PS01155; ATCC PTA-4761) were purchased from ATCC.

Techniques: Isolation

Journal: JCI Insight

Article Title: Glycolytic requirement for NK cell cytotoxicity and cytomegalovirus control

doi: 10.1172/jci.insight.95128

Figure Lengend Snippet: (A) Mice were either depleted of NK cells using α-NK1.1 or treated with IgG2a; they were given 2 doses of ALT-803 and infected with 1 × 105 PFU murine cytomegalovirus (MCMV). 2-Deoxy-ᴅ-glucose (2DG) was given every 24 hours, and mice were euthanized on day 2 for assessment of (B) clearance of m157-transgenic targets from the spleen and (C) viral copy number from the spleen and liver; they were measured by quantitative PCR and log-transformed (n = 10 individuals/group, 2 separate experiments, 2-tailed t test). (D) To assess susceptibility, an additional NK depletion was given at day 3 (red, n = 30); control mice were treated with IgG2a (black, n = 23). Mice were followed for 10 days (3 separate experiments). (E) Mice were infected with MCMV, given daily i.p. injections of 1.5 mg/kg rapamycin (green) or vehicle (black), and monitored for susceptibility (n = 10/group, 1 experiment). (F) ALT-803 was given to MCMV-infected, rapamycin-treated mice on the same schedule as for 2DG-treated mice, with doses at –3, –1, and +2 days relative to infection (n = 10/group, 1 experiment). Data show mean ± SEM or survival in D–F analyzed by log-rank Mantel-Cox test. ***P < 0.001. Asterisks in A and D indicate treatment with ALT-803.

Article Snippet: For NK and CD8 depletion, 100 μg of α-NK1.1 (PK136, BioXcell), 250 μg α-CD8b (53-5.8, BioXcell), or 100 μg murine IgG2a (C1.18.4, BioXcell) was administered i.p. 6 days before MCMV infection, the day of infection, and 3 days after infection.

Techniques: Infection, Transgenic Assay, Real-time Polymerase Chain Reaction, Transformation Assay, Control

Journal: Materials

Article Title: The Osteogenic and Tenogenic Differentiation Potential of C3H10T1/2 (Mesenchymal Stem Cell Model) Cultured on PCL/PLA Electrospun Scaffolds in the Absence of Specific Differentiation Medium

doi: 10.3390/ma10121387

Figure Lengend Snippet: Summary of the main results of morphological, mechanical and biological characterization of the scaffolds seeded with C3H10T1/2 stem cells. Spreading was estimated from the SEM and fluorescence acquisitions as moderate (+), high (++) or very high (+++).

Article Snippet: The murine mesenchymal stem cell line C3H10T1/2 [ ] (ATCC CCL-26) initially proliferated in culture flasks at 37 °C, 5% CO 2 , in 1 g/L glucose DMEM medium (Sigma-Aldrich, St. Louis, MO, USA) complemented with 10% of Fetal Bovine Serum (Gibco Invitrogen, Waltham, MA USA), 2% of penicillin-streptomycin (Gibco Invitrogen, Waltham, MA, USA) and 2% of 200 mM l -glutamine (Gibco Invitrogen, Waltham, MA, USA).

Techniques: Fluorescence